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How to perform test

 Vitamin D | Affimedix 


All you nee​d:

  • 1x Vitamin D Test Cassette
  • 1x 10 µL Fixed-Volume Capillary Pipette
  • 1x Sterile Lancet
  • 1x Alcohol Swab
  • 1x Buffer Solution Tube
  • 1x Igloo Reader
  • 1x Affimedix Adapter

    

STEP 1: Prepare and Disinfect the Sampling Area

Lay out all kit components. Disinfect the fingertip with the alcohol swab and let it dry.

    

STEP 2: Perform a Fingerstick

Use the sterile lancet to puncture the side of the fingertip. Gently massage the finger to form a blood drop.


​

STEP 3: Collect Exactly 10 µL of Blood

Use the provided fixed-volume pipette. Allow it to fill automatically without squeezing. Only use the supplied pipette for accuracy. 

    

STEP 4: Mix Blood with Buffer

Transfer the collected blood into the buffer tube. Mix gently by inverting or swirling several times.

Do not shake vigorously.

   

STEP 5: Insert the Test Cassette into the Adapter

Place the cassette firmly into the Igloo adapter.

Important: The sample well must be on the opposite side of the arrow indicating insertion direction. 

    ​

STEP 6: Add 3 Drops of the Mixed Sample

Use the dropper to dispense 3 full drops into the sample well of the cassette inside the adapter.

Hold vertically. Avoid touching the cassette or creating bubbles.

 

    

STEP 7: Power On the Igloo Reader

Press and hold the power button until the LED activates.


 

    

STEP 8: Start New Measurement & Enter Patient Info

Press "New Measurement" on the touchscreen. Enter patient data (ID, age, etc.).

✅ This info will be linked to the final report.


 

    

STEP 9: Wait 15 Minutes

The reader will start the test. A countdown appears. Do not move or disturb the device.


 

​

STEP 10: Retrieve the Result

When the timer ends, the result is shown on-screen and in the app. Choose an output method:

  • Print via mini-printer
  • Scan the QR code on the reader to get the PDF result to your smartphone


KIT CONTENTS:

  • 1x Vitamin D Test Cassette
  • 1x 10 µL Fixed-Volume Capillary Pipette
  • 1x Sterile Lancet
  • 1x Alcohol Swab
  • 1x Buffer Solution Tube
  • 1x Quick Guide

Note: Igloo Reader and Adapter sold separately.

DISPOSAL:

  • Dispose of used cassettes, pipettes, and lancets as biohazardous waste.
  • Do not reuse any test components.
  • Reader and adapter are reusable; clean according to the official maintenance guide.










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